CST, 82529S, SHANK2 (E2J1N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SHANK2. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, SHANK2 (E2J1N) Rabbit Monoclonal Antibody (CST #82529) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity SHANK2 (E2J1N) Rabbit Monoclonal Antibody recognizes endogenous levels of total SHANK2 protein. This antibody does not cross-react with other SHANK proteins. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro829 of human SHANK2 protein. Background The SHANK family proteins, also known as proline-rich synapse-associated proteins, consist of SHANK1, SHANK2, and SHANK3. SHANK proteins act as scaffolds at the neuronal post-synaptic density (PSD) (1), where they play a critical role in PSD assembly of excitatory synapses during development (2). While recruitment of SHANK proteins to the synapse is independent of their interaction with Homer (3), proper synaptic targeting of SHANK1 is mediated by interactions between its PDZ domain and PSD proteins (4). At the synapse, SHANK proteins interact with NMDA receptors and metabotropic glutamate receptor complexes (5). Research studies have proposed the involvement of SHANK proteins in autism and neurodegenerative diseases (1). Alternate Names AUTS17; cortactin SH3 domain-binding protein; Cortactin-binding protein 1; CORTBP1; CTTNBP1; GKAP/SAPAP interacting protein; KIAA1022; proline-rich synapse associated protein 1; Proline-rich synapse-associated protein 1; PROSAP1; SH3 and multiple ankyrin repeat domains 2; SH3 and multiple ankyrin repeat domains protein 2; SHAN2; SHANK; SHANK2; SHANK2 iso4; SPANK-3 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG