CST, 82637T, SRSF7 (F9G2P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SFRS7. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, SRSF7 (F9G2P) Rabbit Monoclonal Antibody (CST #82637) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SRSF7 (F9G2P) Rabbit Monoclonal Antibody recognizes endogenous levels of total SRSF7 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect isoforms 1-4 of SRSF7. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro86 of human SRSF7 protein. Background The serine/arginine-rich family of splicing factors are crucial regulators in precursor RNA splicing, a process that is associated with maintenance of genome stability, regulation of mRNA export and stability, and translation (1). Serine/arginine-rich splicing factor 7 (SRSF7/SFRS7), also known as splicing factor 9G8, is overexpressed in colon and lung cancer, and regulates proliferation and apoptosis through the splicing of Fas mRNA in human colon and lung cancer cells (2). In human colon cancer cells, SRSF7 knockdown increased levels of the CDK inhibitor p21 Waf1/Cip1 in a p53-independent manner (3). SRSF7 also regulates age-dependent splicing in mice (4) and induces expression of cellular senescence markers in response to DNA damage by generating an alternatively spliced form of p53, p53β (5). Alternate Names 9G8; AAG3; aging-associated protein 3; serine and arginine rich splicing factor 7; Serine/arginine-rich splicing factor 7; SFRS7; Splicing factor 9G8; Splicing factor, arginine/serine-rich 7; splicing factor, arginine/serine-rich 7, 35kDa; SR splicing factor 7; SRSF7 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG