CST, 82669T, TMEM106B (F5O8F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TMEM106B. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, TMEM106B (F5O8F) Rabbit Monoclonal Antibody (CST #82669) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity TMEM106B (F5O8F) Rabbit mAb recognizes endogenous levels of the C-terminal fragment of TMEM106B resulting from cleavage adjacent to Ser120 and full-length TMEM106B protein. Non-specific staining was observed in lung and kidney by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human TMEM106B protein. Background Transmembrane protein 106B (TMEM106B) is a type II transmembrane protein with unknown function (1). was first identified as a major risk factor in genome-wide association studies in patients with frontotemporal lobar degeneration (FTLD), a neurodegenerative disorder characterized by atrophy in the frontal and temporal lobes of the brain (2). Localization of TMEM106B in late endosomal/lysosomal compartments suggests a role in regulating endo-lysosomal morphology and function (3). Endo-lysosomal dysfunction is likely a major cellular pathway that contributes to FTLD as other genetically linked FTLD genes, including , , and , converge on this cellular pathway (4). TMEM106B is sequentially processed through ectodomain shedding, followed by intramembrane proteolysis, with possible variability in the intramembrane cleavage site. Fragments consisting of residues 120-254 of TMEM106B have been shown to form amyloid filaments in human brains (5-7). Alternate Names FLJ11273; HLD16; MGC33727; T106B; TMEM106B; TMEM106B protein; Transmembrane protein 106B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42, 34 Source/Isotype: Rabbit IgG