CST, 82723S, PD-L2 (D7U8C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PDCD1LG2. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, PD-L2 (D7U8C) Rabbit Monoclonal Antibody (CST #82723) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 99337 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity PD-L2 (D7U8C) Rabbit Monoclonal Antibody recognizes endogenous levels of total PD-L2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg168 of human PD-L2 protein. Background Programmed cell death 1 ligand 2 (PD-L2, B7-DC, CD273) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses (1,2). PD-L2 binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L2 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by activated dendritic cells, macrophages, and T cells (1,3). Similar in structure to related B7 family members, PD-L2 protein contains extracellular IgV and IgC2 domains, a transmembrane domain, and a short, cytoplasmic region. Research studies demonstrate that PD-L2 is expressed in several tumor types, including lung cancer, renal cell carcinoma, melanoma, Hodgkin's lymphoma and primary mediastinal large B-cell lymphoma (4-7). Alternate Names B7 dendritic cell molecule; B7-DC; B7DC; bA574F11.2; Btdc; Butyrophilin B7-DC; CD273; MGC142238; MGC142240; PD-1 ligand 2; PD-1-ligand 2; PD-L2; PD1L2; PDCD1 ligand 2; PDCD1L2; PDCD1LG2; PDL2; Programmed cell death 1 ligand 2; Programmed death ligand 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45-60 Source/Isotype: Rabbit IgG