CST, 82746S, Iba1/AIF-1 (E4O4W) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3003)

Monoclonal Antibody for studying AIF1/Iba1. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Iba1/AIF-1 (E4O4W) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3003) (CST #82746) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity Iba1/AIF-1 (E4O4W) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3003) recognizes endogenous levels of total Iba1/AIF-1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala139 of human Iba1/AIF-1 protein. Background Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammatory factor 1 (AIF-1), is an evolutionarily conserved cytoplasmic calcium binding protein containing a central pair of EF-hand calcium binding motifs (1,2). Iba1/AIF-1 was originally cloned from activated macrophages in human atherosclerotic allogenic heart grafts undergoing chronic transplant rejection as well as from rat monocytes (3,4). Its function is not very well understood, but Iba1/AIF-1 expression is upregulated in response to interferon-gamma and, therefore, could modulate macrophage-dependent immune response (3). As an F-actin-binding protein, Iba1/AIF-1 may function to remodel the actin cytoskeleton and contribute to morphological changes that correlate with various microglial/macrophage states (5). Iba1/AIF-1 is also uniquely expressed in cells of monocytic lineage and is, therefore, widely used as a marker for microglia/macrophages in the brain and other tissue (6). Alternate Names AIF-1; AIF1; Allograft inflammatory factor 1; G1; IBA 1; IBA-1; IBA1; interferon gamma responsive transcript; Ionized calcium-binding adapter molecule 1; IRT-1; IRT1; Protein G1 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG