CST, 82838T, EGF (F7W4J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EGF. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, EGF (F7W4J) Rabbit Monoclonal Antibody (CST #82838) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity EGF (F7W4J) Rabbit Monoclonal Antibody recognizes endogenous levels of total full-length EGF protein. The antibody only detects the EGF precursor protein and does not detect the EGF growth factor fragment. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human EGF protein. Background Epidermal growth factor (EGF) is a small polypeptide hormone that has mitogenic properties and and affects the growth and/or differentiation of many cell types. EGF elicits biological responses by binding to its cell surface receptor, which is a transmembrane glycoprotein containing a cytoplasmic protein tyrosine kinase (PTK) (1,2). The binding of EGF to EGF receptor induces dimerization of the receptor, autophosphorylation, and activation of downstream signaling components (3). The integrated biological responses to EGF signaling are pleiotropic, including mitogenesis or apoptosis, enhanced cell motility, protein secretion, and differentiation or dedifferentiation. In addition to being implicated in organ morphogenesis, maintenance, and repair, research studies have correlated upregulated EGF receptor signaling with progression to invasion and metastasis in a wide variety of tumors (4-6). Thus, investigators have identified EGF receptor and its downstream signaling molecules as targets for therapeutic interventions in wound repair and cancer (4-6). EGF is derived from a 160 kDa precursor protein with an N-terminal extracellular domain, an EGF growth factor sequence, a transmembrane domain, and an extracellular tail (7). Once synthesized, the precursor can be stored or processed into EGF growth factor depending on different tissue/organ conditions. Alternate Names beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); HOMG4; Pro-epidermal growth factor; URG; Urogastrone Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Rabbit IgG