CST, 82933S, MT-ND3 (E8O4E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MT-ND3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MT-ND3 (E8O4E) Rabbit Monoclonal Antibody (CST #82933) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MT-ND3 (E8O4E) Rabbit Monoclonal Antibody recognizes endogenous levels of total MT-ND3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MT-ND3 protein. Background Mitochondrially encoded NADH dehydrogenase 3 (MT-ND3) is encoded by the mitochondrial gene and is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). Located in the mitochondrial inner membrane, Complex I is the largest of the five respiratory complexes and catalyzes electron transfer from NADH to ubiquinone. The mitochondrially encoded subunits of Complex I, including MT-ND3, are the most hydrophobic subunits and form the core of the transmembrane region (1,2). Pathogenic variants of the gene are known to cause mitochondrial complex I deficiency (MT-C1D) and may lead to a wide range of clinical disorders, including Leigh syndrome, Leber hereditary optic neuropathy, and encephalopathy (3-5). Alternate Names mitochondrially encoded NADH dehydrogenase 3; MT-ND3; MTND3; NADH dehydrogenase subunit 3; NADH dehydrogenase, subunit 3 (complex I); NADH-ubiquinone oxidoreductase chain 3; NADH3; ND3; NU3M Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 13 Source/Isotype: Rabbit IgG