CST, 82971S, Phospho-BCL2L13 (Ser261) Antibody

Polyclonal Antibody for studying Bcl-2L13 (Ser261) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-BCL2L13 (Ser261) Antibody (CST #82971) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-BCL2L13 (Ser261) Antibody recognizes endogenous levels of BCL2L13 protein only when phosphorylated at Ser261. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser261 of human BCL2L13 protein. Antibodies are purified by peptide affinity chromatography. Background Bcl-2-like protein 13 (BCL2L13), also known as Bcl-rambo, is an atypical member of the Bcl-2 family of proteins that regulates apoptosis at the mitochondria (1). While it contains conserved Bcl-2 homology (BH) domains -1, -2, -3, and -4, it has a unique C-terminal region and does not interact with other Bcl-2 family members (1). BCL2L13 may regulate apoptosis in a cellular context manner (reviewed in 2). It was originally identified as a pro-apoptotic protein with activity dependent on its C-terminal region (1). Interaction with the adenine nucleotide translocator (ANT) suppresses the ADP/ATP-dependent translocation activity of ANT (3). Other studies have found that BCL2L13 can inhibit apoptosis in glioblastoma and tumor growth (4). These studies found that BCL2L13 binds to pro-apoptotic ceramide synthases 2 (CerS2) and 6 (CerS6) and blocks homo- and heteromeric CerS2/6 complex formation and activity. Studies have also found that BCL2L13 can regulate mitochondrial fragmentation and can bind to LC3 to facilitate the clearance of damaged mitochondria through mitophagy (5,6). Phosphorylation of BCL2L13 at Ser275 plays a role in the clearance of damaged mitochondria through mitophagy and is important in maintaining cardiac function (7). Phosphorylation of BCL2L13 at Ser261 and Ser275 occurs following AMPK activation. For more information, visit BioRxiv at https://www.biorxiv.org/content/10.1101/2025.07.10.663832v1. Alternate Names B2L13; Bcl-2-like protein 13; Bcl-2L13; Bcl-rambo; BCL2 like 13; Bcl2-L-13; BCL2-like 13 (apoptosis facilitator); BCL2L13; MIL1; Protein Mil1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90 SOURCE: Rabbit