CST, 82985T, Pancreatic polypeptide (F8J4Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PPY. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Pancreatic polypeptide (F8J4Y) Rabbit Monoclonal Antibody (CST #82985) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Immunofluorescence (Frozen): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Pancreatic polypeptide (F8J4Y) Rabbit Monoclonal Antibody recognizes transfected levels of total pancreatic polypeptide (PP) protein by western blot, and endogenous levels of total PP protein by immunohistochemistry and immunofluorescence. Nonspecific labeling in fixed frozen mouse small intestine and ovary has been observed by immunofluorescence. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro63 of human PP protein. Background The neuropeptide Y (NPY) family of biologically active peptides has three members: NPY, peptide YY (PYY) and pancreatic polypeptide (PP). All peptides consist of 36 amino acids and play a role in the regulation of appetite and food intake (1,2). PYY and PP are almost exclusively expressed at the digestive system level, whereas NPY is found at all levels of the gut-brain and brain-gut axis. PP is postprandially secreted from the pancreas, where it is synthesized by endocrine F cells of the pancreatic islets (1,3). The primary role of PP is to modulate digestion of food by inhibition of gastric emptying as well as biliary secretion (4). PP binds to its constitutive Y4 receptor that is expressed in the liver, intestine, adrenal gland and parts of the central nervous system (5). Alternate Names PAHO; Pancreatic hormone; Pancreatic icosapeptide; Pancreatic polypeptide; Pancreatic polypeptide prohormone; pancreatic polypeptide Y; Pancreatic prohormone; PH; PI; PNP; PP; PPY; prepro-PP (prepropancreatic polypeptide) Specification REACTIVITY: H M SENSITIVITY: Endogenous (IHC-P, IF-F), Transfected (W) MW (kDa): 15 Source/Isotype: Rabbit IgG