CST, 83000T, MAVS (E8Z7M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MAVS mouse. Validated for WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, MAVS (E8Z7M) Rabbit Monoclonal Antibody (CST #83000) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 98156 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MAVS (E8Z7M) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAVS protein. Non-specific nuclear staining in mouse brain and apical membrane staining of mouse stomach epithelium were observed by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala449 of mouse MAVS protein. Background The mitochondrial antiviral signaling protein (MAVS, VISA) contributes to innate immunity by triggering IRF-3 and NF-κB activation in response to viral infection, leading to the production of IFN-β (1). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. The MAVS adaptor protein plays a critical and specific role in viral defenses (2). MAVS acts downstream of the RIG-I RNA helicase and viral RNA sensor, leading to the recruitment of IKKε, TRIF, and TRAF6 (3,4). Some viruses have evolved strategies to circumvent these innate defenses by using proteases that cleave MAVS to prevent its mitochondrial localization (5,6). Alternate Names CARD adapter inducing interferon beta; CARD adapter inducing interferon-beta; Cardif; D430028G21Rik; IFN-beta promoter stimulator-1; Interferon beta promoter stimulator protein 1; interferon-beta promoter stimulator protein 1; IPS-1; Ips1; Mavs; MGC25836; mitochondrial anti-viral signaling protein; mitochondrial antiviral signaling protein; Mitochondrial antiviral-signaling protein; OTTMUSP00000017159; OTTMUSP00000017160; virus-induced signaling adapter; Virus-induced-signaling adapter; Visa Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG