CST, 83020S, PEX5 (D7V4D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PEX5. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PEX5 (D7V4D) Rabbit Monoclonal Antibody (CST #83020) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PEX5 (D7V4D) Rabbit Monoclonal Antibody recognizes endogenous levels of total PEX5 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe326 of human PEX5 protein. Background Peroxin-5 (PEX5) is the shuttle receptor that delivers proteins to peroxisomes (1). In the cytosol, PEX5 binds to the peroxisomal targeting signal 1 (PTS1), a short peptide sequence present at the extreme C termini of newly synthesized peroxisomal matrix proteins. The PEX5-cargo complex interacts with the peroxisomal docking/translocation machinery on the peroxisomal membrane, where the cargo is released into the organelle matrix. During this process, PEX5 is monoubiquitinated at a conserved cysteine residue, and the ubiquitin-PEX5 conjugate is released from the organelle into the cytosol, where PEX5 is deubiquitinated and ready the next round of targeting (2,3). Alternate Names FLJ50634; FLJ50721; FLJ51948; PBD2A; PBD2B; Peroxin-5; peroxisomal biogenesis factor 5; Peroxisomal C-terminal targeting signal import receptor; peroxisomal import receptor 5; peroxisomal targeting signal 1 (SKL type) receptor; Peroxisomal targeting signal 1 receptor; peroxisomal targeting signal import receptor; peroxisomal targeting signal receptor 1; Peroxisome receptor 1; PEX5; PTS1 receptor; PTS1-BP; PTS1R; PXR1; RCDP5 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG