CST, 83029T, EphB2 (D2X2I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EphB2. Validated for WB,WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, EphB2 (D2X2I) Rabbit Monoclonal Antibody (CST #83029) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 Immunofluorescence (Immunocytochemistry): 1:600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 87529 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity EphB2 (D2X2I) Rabbit Monoclonal Antibody recognizes endogenous levels of total EphB2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala573 of human EphB2 protein. Background The ephrin receptor B2 (EphB2) is an ephrin family receptor tyrosine kinase that plays an important role in regulating growth and development of multiple tissues and organs (1,2). The EphB2 transmembrane receptor protein contains a kinase domain, a PDZ motif, and a SAM domain within a conserved cytoplasmic domain. A ligand binding domain, a cysteine-rich domain, and fibronectin type III repeats comprise the conserved EphB2 extracellular domain (3). EphB2 binds with high affinity to ephrin B ligands, and to some ephrin A proteins, to initiate bidirectional signaling between neighboring cells (1,2). Upon binding, EphB2-Ephrin B2 dimers form a heterotetramer and position the receptor-ligand complex on the cell membrane to facilitate bidirectional signal transduction (3). In addition to associating with ephrin ligands, EphB2 also regulates a number of biological processes through interaction with focal adhesion kinase (FAK), NMDA receptor (NMDAR), the Rac1 guanine nucleotide exchange factor Tiam1, and p21-activated kinase (PAK1) (4-7). While some studies support a role for EphB2 as a pro-oncogenic kinase, other research suggests that EphB2 acts as a tumor suppressor (1,2,4,8). Alternate Names BDPLT22; CAPB; Developmentally-regulated Eph-related tyrosine kinase; DRT; EK5; elk-related tyrosine kinase; EPH receptor B2; eph tyrosine kinase 3; EPH-like kinase 5; EPHB2; EphB2/CTF1; EphB2/CTF2; Ephrin type-B receptor 2; EPHT3; EPTH3; ERK; HEK5; MGC87492; PCBC; protein-tyrosine kinase HEK5; Renal carcinoma antigen NY-REN-47; TYRO5; Tyrosine-protein kinase receptor EPH-3; Tyrosine-protein kinase TYRO5 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG