CST, 83034T, PYGO2 (F7N8Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PYGO2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PYGO2 (F7N8Z) Rabbit Monoclonal Antibody (CST #83034) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PYGO2 (F7N8Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total PYGO2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PYGO2 protein. Background Stem cells are important for tissue homeostasis and regeneration and can serve as originating cells for some cancers. Understanding how their differentiation is regulated by epigenetic programs and signaling at the cell membrane should help elucidate the biology of heterogeneous tumor cells (1-3). The Pygopus (PYGO) family of proteins was first discovered in and later in (1-3). Of the two human PYGO homologs, PYGO2 is more widely expressed and functionally characterized (3). Genetic studies have shown PYGO2 as a component of canonical Wnt signaling (1-3). With a highly conserved plant homeodomain (PHD) in its C-terminus, PYGO2 binds epigenetic marks and activates gene expression. Evidence suggests that PYGO2 modulates transcription through both Wnt and Notch pathways (2-3). Dysregulation and increased expression of PYGO2 have been observed in several malignancies: breast (4,5), ovarian, colon (6,7), and prostate (8,9). The role of PYGO2 in immuno-oncology is being actively investigated. Loss of PYGO2 results in increased activation and infiltration of cytotoxic T lymphocytes (CTLs) and sensitizes tumor cells to killing (9). Increased PYGO2 has been shown to result in an aberrant tumor microenvironment hostile to CTLs. Pharmacological targeting of PYGO2 enhances the efficacy of immunotherapies when combined with immune checkpoint blockade (ICB) (9). Alternate Names 1190004M21Rik; FLJ33226; PYGO2; pygopus 2; pygopus family PHD finger 2; Pygopus homolog 2; pygopus homolog 2 (Drosophila) Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 50-60 Source/Isotype: Rabbit IgG