CST, 8311T, PTPN18 (D7B9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PTPN18. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PTPN18 (D7B9) Rabbit Monoclonal Antibody (CST #8311) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PTPN18 (D7B9) Rabbit Monoclonal Antibody recognizes endogenous levels of total PTPN18 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala401 of human PTPN18 protein. Background PTPN18 is a member of the PEST family of nonreceptor protein tyrosine phosphatases, a group that also includes PTP-PEST and PEP (1-3). Members of this protein family contain an N-terminal catalytic domain and a noncatalytic C-terminal domain with a PEST motif that mediates protein-protein interactions (4). PTPN18 was first identified in hematopoietic stem cells (HSCs), where it was suggested to play a role in HSC differentiation (1-3). Subsequently, PTPN18 was shown to bind to c-Src kinase, forming a protein complex that functions to inhibit Src-family kinase signaling (4,5). More recently, PTPN18 expression has been shown to correlate with HER2 receptor expression in breast cancer cell lines (6,7), where it has been suggested to repress HER2 signaling (7). Alternate Names BDP1; Brain-derived phosphatase; protein tyrosine phosphatase non-receptor type 18; protein tyrosine phosphatase, non-receptor type 18 (brain-derived); PTN18; PTP-HSCF; PTPN18; Tyrosine-protein phosphatase non-receptor type 18 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG