CST, 83157SF, FAM3C (D1S2D) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying FAM3C. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, FAM3C (D1S2D) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #83157) is ready to ship. Product Usage Information This product is the carrier free version of product #15171. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 15171 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity FAM3C (D1S2D) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total FAM3C protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with other FAM3 family members. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly169 of human FAM3C protein. Background FAM3C, also known as ILEI (interleukin-like epithelial-to-mesenchymal transition [EMT] inducer), is a cytokine-like protein and member of the FAM3 family. FAM3C plays an important role in EMT and metastasis during cancer progression in human and mouse cells, and is highly expressed in human cancer (1,2). In colorectal cancer, researchers have indicated that FAM3C is a marker for EMT and tumor progression, and that high expression of FAM3C is predictive of poor prognosis (3). While EMT induction by FAM3C can be independent of TGF-beta, research studies have also shown TGF-beta-dependent regulation of FAM3C expression at the translational level in mouse and human cells (4,5). FAM3C has also been linked to regulation of osteoblast differentiation (6), and to accumulation of amyloid beta plaques in Alzheimer's disease (7). FAM3C exists in monomeric and in homodimeric form, and research shows that FAM3C homodimers contain its EMT-inducing and tumor promoting activity (8). Alternate Names FAM3 metabolism regulating signaling molecule C; FAM3C; family with sequence similarity 3 member C; family with sequence similarity 3, member C; GS3786; ILEI; interleukin-like EMT inducer; interleukin-like epithelial-mesenchymal transition inducer; predicted osteoblast protein; Protein FAM3C Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG