CST, 83159S, T7-Tag (F1O6G) Mouse Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, T7-Tag (F1O6G) Mouse Monoclonal Antibody (CST #83159) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity T7-Tag (F1O6G) Mouse Monoclonal Antibody detects recombinant proteins containing the T7 epitope tag. This antibody recognizes the T7-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody detects exogenously expressed T7-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody may detect several non-specific bands of unknown origin in rat cell lines. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic T7-tag peptide. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The T7-tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene 10. The T7-tag has been used extensively as a general epitope tag in many expression vectors, including the pET system that is based on T7 RNA polymerase expression systems (1). Specification REACTIVITY: All SENSITIVITY: Recombinant and Transfected Source/Isotype: Mouse IgG1 kappa