CST, 83186T, Cleaved-IL-1 beta (Asp116) (D3A3Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IL1 beta (Asp116) cleaved. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved-IL-1 beta (Asp116) (D3A3Z) Rabbit Monoclonal Antibody (CST #83186) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Cleaved-IL-1 beta (Asp116) (D3A3Z) Rabbit Monoclonal Antibody recognizes endogenous levels of mature IL-1β protein only when cleaved at Asp116. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp116 of human IL-1β protein. Background Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity. Alternate Names Catabolin; IL-1; IL-1 beta; IL1-BETA; IL1B; IL1beta; IL1F2; interleukin 1 beta; interleukin 1, beta; interleukin 1beta; Interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 17 Source/Isotype: Rabbit IgG