CST, 83197SF, Basic FGF (E5Y6M) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying FGF2 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Basic FGF (E5Y6M) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #83197) is ready to ship. Product Usage Information This product is the carrier free version of product #46879. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 46879 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Basic FGF (E5Y6M) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total basic FGF protein. Species cross-reactivity for IF is human only. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His24 of mouse basic FGF protein. Background Fibroblast growth factors are a family of broad-spectrum growth factors influencing a plethora of cellular activities. The interaction of at least 23 ligands, four receptors, and multiple coreceptors provides a dramatic complexity to a signaling system capable of effecting a multitude of responses (1,2). Basic fibroblast growth factor (bFGF or FGF2), initially identified as a mitogen with prominent angiogenic properties, is now recognized as a multifunctional growth factor (3). It is clear that bFGF produces its biological effects in target cells by signaling through cell-surface FGF receptors. bFGF binds to all four FGF receptors. Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic downstream target proteins, including FRS-2, PLC, and Crk (4,5). The FGF signaling pathway appears to play a significant role not only in normal cell growth regulation but also in tumor development and progression (6).Acidic FGF (aFGF or FGF1) is another extensively investigated protein of the FGF family. aFGF shares 55% DNA sequence homology with bFGF. These two growth factors are ubiquitously expressed and exhibit a wide spectrum of similar biological activities with quantitative differences likely due to variations in receptor affinity or binding (7). Alternate Names Basic fibroblast growth factor; BFGF; Fgf-2; Fgf2; Fgfb; Fibroblast growth factor 2; HBGF-2; Heparin-binding growth factor 2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 18, 20, 22, 24 Source/Isotype: Rabbit IgG