CST, 83200T, AhR (D5S6H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying AhR. Validated for WB,IP,ChIP,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, AhR (D5S6H) Rabbit Monoclonal Antibody (CST #83200) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:50 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP, CUT&RUN Specificity / Sensitivity AhR (D5S6H) Rabbit Monoclonal Antibody recognizes endogenous levels of total AhR protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AhR protein. Background The aryl hydrocarbon receptor (AhR) is a ligand activated transcription factor involved in xenobiotic metabolism, cell cycle regulation, and development in response to both endogenous and environmental signals (1,2). AhR was initially identified as a receptor for dioxins, which are environmental pollutants generated by waste incineration and other industrial processes (3,4). AhR ligands include polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene and other components of cigarette smoke (3,4). Naturally occurring AhR ligands include flavonoids, which are aromatic plant secondary compounds commonly found in vegetables and fruits (3). Cytoplasmic aryl hydrocarbon receptors are found in protein complexes with heat shock proteins. Upon ligand binding, AhR dissociates from heat shock proteins and translocate to the nucleus where it dimerizes with AhR nuclear translocator (ARNT, HIF-1β). The AhR/ARNT heterodimer binds to nuclear xenobiotic response elements to control the expression of genes associated with xenobiotic metabolism, including several cytochrome P450 genes (5,6). AhR is ubiquitously expressed and is thought to play a role in regulation of cell proliferation and differentiation, cytokine expression, and xenobiotic metabolism (2). Research studies link AhR activity with the control of regulatory T-cell and T-helper 17 cell differentiation, regulation of the inflammatory response, and the onset of lung cancer (1,2,7,8). Alternate Names Ah receptor; AH-receptor; AHR; aromatic hydrocarbon receptor; Aryl hydrocarbon receptor; BHLHE76; Class E basic helix-loop-helix protein 76; RP85 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG