CST, 83243SF, ZEB1 (E2G6Y) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ZEB1/TCF8. Validated for WB,IHC,IHC,IF,F. Highly specific and rigorously validated in-house, ZEB1 (E2G6Y) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #83243) is ready to ship. Product Usage Information This product is the carrier-free version of product #70512. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user.BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 70512 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles . A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ZEB1 (E2G6Y) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ZEB1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the central region of human ZEB1 protein. Background ZEB family proteins are zinc-finger and homeobox domain containing transcription factors. There are two members in mammals, ZEB1 (δ-EF1, TCF8, AREB6) and ZEB2 (SIP1). ZEB1 and ZEB2 contain two separate zinc-finger domains and a homeodomain (1). While ZEB proteins mainly function as transcriptional suppressors, they are able to activate transcription, dependent on DNA-context and cell type (1). One of the targets suppressed by ZEB proteins is E-cadherin. Downregulation of E-cadherin is one of the hallmarks of epithelial mesenchymal transition (EMT), a critical feature of normal embryonic development, which is also utilized by malignant epithelial tumors to spread beyond their origin (2-4). ZEB1 mutations are associated with posterior corneal dystrophy, and ZEB2 mutations were reported to be associated with Hirschsprung (HSCR) disease (5-8). Alternate Names AREB6; BZP; delta-crystallin enhancer binding factor 1; DELTAEF1; FECD6; MGC133261; Negative regulator of IL2; NIL-2-A zinc finger protein; NIL2A; posterior polymorphous corneal dystrophy 3; PPCD3; TCF-8; TCF8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); ZEB1; ZFHEP; ZFHX1A; zinc finger E-box binding homeobox 1; Zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG