CST, 83259T, p19 ARF (F6F7N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying p19 Arf. Validated for WB,IP,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, p19 ARF (F6F7N) Rabbit Monoclonal Antibody (CST #83259) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:500 - 1:2000 Flow Cytometry (Fixed/Permeabilized): 1:500 - 1:2000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity p19 ARF (F6F7N) Rabbit Monoclonal Antibody recognizes endogenous levels of total p19 ARF protein. This antibody does not recognize p16 INK4A protein or human p14 ARF protein. This antibody detects a 60 kDa and a 160 kDa protein of unknown identity. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of mouse p19 ARF protein. Background Human p14 ARF (p19 ARF in mouse) is a pro-apoptotic cell cycle regulator frequently inactive in human tumors (1). Basal expression of p14 ARF is low in most cell types, but accumulation of this protein occurs in response to oncogene expression (2,3). Increased p14 ARF levels facilitate MDM2 degradation, leading to increased p53 protein levels and subsequent cell cycle arrest and/or apoptosis (4). While most p14 ARF signaling has traditionally thought to be p53-dependent, more recent reports have described p53-independent p14 ARF signaling leading to cell cycle arrest and apoptosis (5,6). Alternate Names Alternative reading frame; ARF; ARF-; ARF-INK4a; CDK4I; Cdkn2a; cyclin dependent kinase inhibitor 2A; cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor protein; INK4; INK4a-ARF; Ink4a/Arf; mitochondrial smARF; MTS; MTS1; p16; p16-INK4; p16-INK4a; p16-INK4A iso3; p16(INK4a); p16I; p16INK4a; p19; p19; p19ARF; Pct; Pctr1; Tumor suppressor ARF Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 21 Source/Isotype: Rabbit IgG