CST, 83312S, SMG5 (F9P6O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SMG5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SMG5 (F9P6O) Rabbit Monoclonal Antibody (CST #83312) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SMG5 (F9P6O) Rabbit Monoclonal Antibody recognizes endogenous levels of total SMG5 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro598 of human SMG5 protein. Background Nonsense-mediated mRNA decay (NMD) is a process by which prematurely terminated transcripts are degraded before they can be translated (1-3). There are two differential processes for NMD in higher eukaryotes, endonucleolytic and exonucleolytic, which are governed by SMG6 (also known as EST1A) or SMG5 and SMG7, respectively (1,4-8). SMG5, SMG6, and SMG7 are structurally similar, but SMG6 contains a PIN domain with endonucleolytic activity. SMG5 contains a similar domain, but lacks critical residues for endonuclease activity. SMG7 does not contain a PIN domain (7). All three proteins are recruited to UPF1 when SMG1 phosphorylates it, and the NMD process significantly diverges later. Upon binding to UPF1, SMG5 and SMG7 can recruit decaying and deadenylating protein complexes, resulting in subsequent mRNA degradation via the XRN1 exonuclease and the exosome (8,9). SMG6, however, promotes decay through cleaving nonspecific sites within 40 nucleotides of the premature termination codon (PTC) (7,10). Interestingly, SMG6-mediated decay depends on either a functional SMG5 or SMG7 (11). NMD pathways are species-specific, with relying more on SMG6-mediated decay and yeast utilizing the decaying and deadenylating approach to mRNA turnover (12). Alternate Names EST1 telomerase component homolog B; EST1-like protein B; EST1B; Est1p-like protein B; ever shorter telomeres 1B; FLJ34864; hSMG-5; KIAA1089; LPTS interacting protein; LPTS-interacting protein; LPTS-RP1; LPTSRP1; Nonsense-mediated mRNA decay factor SMG5; Protein SMG5; RP11-54H19.7; SMG-5; SMG-5 homolog; smg-5 homolog, nonsense mediated mRNA decay factor; Smg-5 homolog, nonsense mediated mRNA decay factor (C. elegans); SMG5; SMG5 nonsense mediated mRNA decay factor Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 145 Source/Isotype: Rabbit IgG