CST, 83424S, Granzyme C (E5M3N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Granzyme C mouse. Validated for Western Blotting,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Granzyme C (E5M3N) Rabbit Monoclonal Antibody (CST #83424) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:800 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 10352 . Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen) Specificity / Sensitivity Granzyme C (E5M3N) Rabbit Monoclonal Antibody recognizes endogenous levels of total Granzyme C protein. This antibody does not cross-react with other Granzyme proteins. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val190 of mouse Granzyme C protein. Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). In mice, ten family members have been identified: A, B, C, D, E, F, G, K, M, and N (2). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein (3). Granzyme C is the closest murine homolog of human Granzyme H and is located directly downstream of (4). Granzyme C has been demonstrated to induce cell death with a similar potency as Granzyme B, and CTLs from mice with reduced expression of Granzyme C and F display diminished cytotoxicity (5). Granzyme C has been identified as a marker for type 1 innate lymphoid cells (ILC1s) exhibiting cytotoxic effector function in addition to ‘helper-like' function (6). These cells have been shown to regulate antitumor immunity and autoimmunity in mouse models (6). Alternate Names AI323531; B10; CCP; CCP2; Ctl; Ctla; Ctla-5; Ctla5; Cytotoxic cell protease 2; GRAC; Granzyme C; Gzmc Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG