CST, 83458T, ECM1 (E3B9A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ECM1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, ECM1 (E3B9A) Rabbit Monoclonal Antibody (CST #83458) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity ECM1 (E3B9A) Rabbit Monoclonal Antibody recognizes endogenous levels of total ECM1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human ECM1 protein. Background Extracellular matrix gene 1 (ECM1) is a secreted glycoprotein found primarily in the extracellular matrix. It acts as both a multifunctional binding core and as a scaffolding protein that interacts with a variety of extracellular and structural proteins, including perlecan, fibulin-1C/1D, and MMP-9. (1). Research studies have shown that ECM1 is involved in the maintenance of tissue integrity and homeostasis, T cell immune responses, and angiogenesis (2-5). Its importance in maintaining tissue integrity is demonstrated by reports showing that mutations in the gene encoding ECM1 lead to skin lipoid proteinosis (1). Notably, ECM1 expression is upregulated in a variety of cancers, and during metastasis, suggesting a role for ECM1 in promoting tumor cell proliferation and invasion (6). In this context, research studies have shown that ECM1 can activate various signaling pathways, such as integrin/FAK, MMP-9/galectin-3, and S100A4/RhoA (7-9). In hepatic tissues, it was furthermore shown that ECM1 contributes to maintaining the latency of TGF-β in the extracellular matrix, thereby preventing spontaneous TGF-β activation and liver fibrosis (10,11). Alternate Names ECM1; Extracellular matrix protein 1; Secretory component p85; testicular tissue protein Li 61; URBWD Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 75-100 Source/Isotype: Rabbit IgG