CST, 83535S, WT1 (D8I7F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying WT1. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, WT1 (D8I7F) Rabbit Monoclonal Antibody (CST #83535) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity WT1 (D8I7F) Rabbit Monoclonal Antibody recognizes endogenous levels of total WT1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human WT1 protein. Background Wilms' Tumor 1 (WT1) is a transcription factor named from Wilms' Tumor 1, an embryonal malignancy of the kidneys that is caused by mutations in the gene (1). It is highly important in development, particularly of the genitourinary system, and mutations and dysregulation of WT1 expression result in a variety of syndromes affecting the genitourinary system and other tissues (2-5). WT1 has a myriad of biological functions and a host of interacting partners and target genes (6). It can behave as a transcriptional activator, or a repressor, and can act as an oncogene or a tumor suppressor (7). It exerts influence over the epigenetic landscape, and also has post-translational influence of gene expression through RNA interactions (8). The diverse biological roles of WT1 have been attributed to the existence of multiple isoforms and post-translational modifications of the protein (9). Alternate Names AWT1; GUD; NPHS4; WAGR; Wilms tumor 1; Wilms tumor protein; WIT-2; WT1; WT1 transcription factor; WT33 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 51-60 Source/Isotype: Rabbit IgG