CST, 83559SF, Cyclin E1 (D7T3U) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Cyclin E1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cyclin E1 (D7T3U) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #83559) is ready to ship. Product Usage Information This product is the carrier free version of product #20808. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 20808 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Cyclin E1 (D7T3U) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total cyclin E1 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect all isoforms of cyclin E1. This antibody does not cross-react with cyclin E2. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly351 of human cyclin E1 protein. Background Cyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. When conditions are favorable for cell cycle progression, cyclin D/CDK4/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E/CDK2 (1,2). Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites including Thr62, Ser88, Ser72, Thr380, and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7). Alternate Names CCNE; CCNE1; cyclin E1; cyclin Es; cyclin Et; G1/S-specific cyclin-E1; pCCNE1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG