CST, 83578S, Cathepsin G Antibody

Polyclonal Antibody for studying Cathepsin G. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cathepsin G Antibody (CST #83578) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cathepsin G Antibody recognizes endogenous levels of total Cathepsin G protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro130 of human Cathepsin G protein. Antibodies are purified by peptide affinity chromatography. Background Cathepsin G is a serine protease which was first identified in the cytoplasmic azurophilic granules of neutrophil leukocytes (1). As a degradative enzyme, Cathepsin G acts both intracellularly to degrade ingested host pathogens and extracellularly to breakdown ECM components at inflammatory sites (2). Independent of its serine protease activity, Cathepsin G is also known to exert broad-spectrum antibacterial action against Gram-negative and -positive bacteria (3). Cathepsin G has been reported to play an important role in the pathophysiology of several diseases, such as chronic obstructive pulmonary disease (COPD), rheumatoid arthritis, cystic fibrosis, and other conditions clinically manifested by excessive inflammatory reactions (4-7). Because of its role in host defense and disease, Cathepsin G is of interest as a potential therapeutic target (8,9). Alternate Names CATG; Cathepsin G; CG; CTSG; MGC23078 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 29 SOURCE: Rabbit