CST, 83580T, Phospho-CD79B (Tyr207) (F1L6E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD79B (Tyr207) phosphate. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-CD79B (Tyr207) (F1L6E) Rabbit Monoclonal Antibody (CST #83580) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-CD79B (Tyr207) (F1L6E) Rabbit Monoclonal Antibody recognizes endogenous levels of CD79B protein only when phosphorylated at Tyr207. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr207 of human CD79B protein. Background The B-cell antigen receptor (BCR) is composed of membrane immunoglobulin molecules non-covalently associated with the heterodimeric signaling component, CD79A and CD79B (also known as Igα and Igβ, respectively) (1,2). The presence of this receptor complex is essential for B cell development and function (3). Following antigen binding, CD79A/CD79B heterodimers are phosphorylated and initiate intracellular signaling through Src family kinases, Lyn, Blk, and Fyn, as well as Syk and Btk tyrosine kinases (4,5). The complexity of BCR signaling results in a variety of distinct cellular functions, such as proliferation, tolerance, apoptosis, and differentiation (6). BCR-antigen ligation also leads to internalization of the complex, trafficking to late endosomes, and antigen presentation in major histocompatibility molecules on the B cell surface (7,8). CD79B enhances the phosphorylation of CD79A (9). Alternatively spliced transcript variants encoding different isoforms of CD79B have been identified (10). CD79B is widely expressed on B cell malignancies and may serve as a target for therapeutic intervention (11,12). Alternate Names AGM6; B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; B29; CD79B; CD79B antigen; CD79b antigen (immunoglobulin-associated beta); CD79b molecule; CD79b molecule, immunoglobulin-associated beta; Ig-beta; IGB; Immunoglobulin-associated B29 protein Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35-55 Source/Isotype: Rabbit IgG