CST, 83586S, Human Natural Killer Cell Markers Flow Cytometry Panel

Assay Kit for studying CD3epsilon/CD16/FCgR3a/CD45/NCAM1 in the research area. Product Usage Information All antibodies in this kit are compatible with the Flow Cytometry, Live Cell Protocol for Directly Conjugated Antibodies and can be used in a single staining mix. After antibody staining and prior to acquisition on a flow cytometer, we recommend adding Propidium Iodide or 7-AAD to enable identification and exclusion of dead cells from the analysis. Gating strategy for identifying human natural killer cells: If Propidium Iodide or 7-AAD was used, first gate on viable cells. Next, gate based on CD45 expression, then gate on lymphocytes based on forward scatter and side scatter on CD45+ fraction. NK cells are CD3-CD56+, and these cells can further be analyzed for CD16 expression. Storage Antibodies are supplied in 10 mM NaH 2 PO 4 , 150 mM NaCl, 0.09% NaN 3 , 0.1% gelatin, pH7.2. Store at 4 o C. Do not aliquot the antibodies. Protect from light. Do not freeze. All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific "Best By" dates for each individual component. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity Each antibody in the Human Natural Killer Cell Markers Flow Cytometry Panel detects endogenous levels of its target protein and epitopes within the extracellular domains. Species Reactivity: Human Source / Purification