CST, 83667S, Mitofusin-2 (D2D10) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying Mitofusin-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Mitofusin-2 (D2D10) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #83667) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Mitofusin-2 (D2D10) Rabbit Monoclonal Antibody (HRP Conjugate) recognizes endogenous levels of total mitofusin-2 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val573 of human mitofusin-2 protein. Background Mitofusins are mitochondrial transmembrane GTPases that function to regulate mitochondrial fusion, a process that occurs in concert with mitochondrial division and is necessary for the maintenance of structural and genetic mitochondrial integrity (1,2). Two mitofusins have been described in mammals, mitofusin-1 and -2, which share 60% amino acid identity and appear to function coordinately to regulate mitochondrial fusion (3). Mitochondrial fusion is widely recognized as important for normal cell growth and development (4), and may have evolved as a mechanism to offset the deleterious effects of mtDNA mutations (3). Null mutations in either mitofusin are embryonic lethal in mice, whereas conditional knockout studies have shown that combined deletion of mitofusin-1 and mitofusin-2 in skeletal muscle results in severe mitochondrial dysfunction (3). Alternate Names CMT2A; CMT2A2; CMT2A2A; CMT2A2B; CPRP1; HMSN6A; HSG; hyperplasia suppressor; KIAA0214; MARF; MFN2; mitochondrial assembly regulatory factor; mitofusin 2; Mitofusin-2; Transmembrane GTPase MFN2 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG