CST, 83683T, Myelin Basic Protein (E9P7U) Mouse Monoclonal Antibody

Monoclonal Antibody for studying MBP. Validated for Western Blotting,Simple Western™,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Myelin Basic Protein (E9P7U) Mouse Monoclonal Antibody (CST #83683) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunofluorescence (Frozen): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen) Specificity / Sensitivity Myelin Basic Protein (E9P7U) Mouse Monoclonal Antibody recognizes endogenous levels of total MBP protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg188 of human MBP protein. Background Myelin basic protein (MBP) is an abundant central nervous system (CNS) myelin membrane protein that plays an important role in nerve myelination. Myelin sheaths are multi-layered membranes derived from oligodendrocytes that increase the conduction velocity of axonal impulses. MBP helps to adhere the cytoplasmic leaflets of adjacent oligodendrocyte membranes to one another. Several splice variants of MBP are expressed in cells and the protein is modified following translation (i.e., phosphorylation, methylation), which suggests additional membrane adhesion functions (1). Alternate Names Golli-MBP; MBP; MGC99675; Myelin A1 protein; Myelin basic protein; Myelin membrane encephalitogenic protein; proline-rich proteoglycan 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 12-18 Source/Isotype: Mouse IgG2b kappa