CST, 83711SF, Survivin (6E4) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Survivin. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Survivin (6E4) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #83711) is ready to ship. Product Usage Information This product is the carrier free version of product #2802. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2802 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Survivin (6E4) Mouse Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of human survivin. This antibody does not cross-react with other types of apoptotic inhibitor proteins. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human survivin. Background Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6). Alternate Names API4; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat containing 5; baculoviral IAP repeat-containing 5; Baculoviral IAP repeat-containing protein 5; BIRC5; EPR-1; IAP4; Survivin; survivin variant 3 alpha Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 16 Source/Isotype: Mouse IgG1