CST, 83775S, CISD1/mitoNEET (D5M4C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZCD1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CISD1/mitoNEET (D5M4C) Rabbit Monoclonal Antibody (CST #83775) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CISD1/mitoNEET (D5M4C) Rabbit Monoclonal Antibody recognizes endogenous levels of total CISD1/mitoNEET protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human CISD1/mitoNEET protein. Background CDGSH iron-sulfur domain-containing protein 1 (CISD1), also known as mitochondrial outer membrane iron-sulfur (2Fe-2S) protein (mitoNEET) was first identified as the target for the drug pioglitazone, used to treat diabetes (1-3). CISD1/mitoNEET regulates both mitochondrial iron transport into the matrix and mitochondrial respiratory capacity. It has also been shown to affect the dynamics of cellular and whole-body lipid homeostasis (2,4). Furthermore, research studies have shown that CISD1/mitoNEET is overexpressed in human epithelial breast cancer cells and it has been considered a potential chemotherapeutic target (6). Alternate Names C10orf70; CDGSH iron sulfur domain 1; CDGSH iron sulfur domain-containing protein 1; CDGSH iron-sulfur domain-containing protein 1; CISD1; MDS029; MGC14684; MitoNEET; ZCD1; zinc finger CDGSH-type domain 1; zinc finger, CDGSH-type domain 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 12 Source/Isotype: Rabbit IgG