CST, 83805S, ALDH1A2 Antibody

Polyclonal Antibody for studying ALDH1A2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, ALDH1A2 Antibody (CST #83805) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ALDH1A2 Antibody recognizes endogenous levels of total ALDH1A2 protein. Based on sequence comparisons, this antibody is not predicted to cross-react with ALDH1A1. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly350 of human ALDH1A2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The aldehyde dehydrogenase family is a large group of enzymes that catalyze the oxidization of aldehydes into carboxylic acids (1). Aldehyde Dehydrogenase 1A2 (ALDH1A2, RALHD2) is among a group of aldehyde dehydrogenases that catalyze the metabolism of retinaldehyde into retinoic acid (RA), which plays a critically important signaling role in animal development (2). Research studies have shown that ALDH1A2 also plays a role postnatally in modulating the effects of RA signaling on immune cell function (3-5). In one example using a genetic mouse model, it was shown that ALDH1A2-dependent RA signaling was a downstream mediator of NOTCH-dependent T cell differentiation (6). Alternate Names AL1A2; aldehyde dehydrogenase 1 family member A2; aldehyde dehydrogenase 1 family, member A2; Aldehyde dehydrogenase family 1 member A2; ALDH1A2; MGC26444; RALDH 2; RALDH(II); RALDH2; RALDH2-T; Retinal dehydrogenase 2; Retinaldehyde-specific dehydrogenase type 2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 55 SOURCE: Rabbit