CST, 83811S, PHOX2B Antibody

Polyclonal Antibody for studying PHOX2B. Validated for Western Blotting. Highly specific and rigorously validated in-house, PHOX2B Antibody (CST #83811) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PHOX2B Antibody recognizes endogenous levels of total PHOX2B protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro284 of human PHOX2B protein. Antibodies are purified by peptide affinity chromatography. Background PHOX2B (also called neuroblastoma PHOX, NBPhox) is a homeodomain transcription factor expressed primarily in neuronal cells of the peripheral autonomic nervous system (1). Research studies in animal models have shown that PHOX2B expression is specifically required for the generation and maintenance of neuroblasts derived from the neural crest (2). PHOX2B expression has likewise been identified as a sensitive and specific marker of undifferentiated neuroblastoma tumors, including metastatic neuroblastoma cells in bone marrow (3-5). Specific mutations in the gene encoding PHOX2B have been directly linked with familial neuroblastoma cases (6) in addition to autonomic nerve disorders that can co-occur with neuroblastoma (e.g., Hirschsprung's disease, congenital central hypoventilation syndrome) (7). These findings underscore the central importance of PHOX2B to development and function of the peripheral autonomic nervous system. Alternate Names CCHS; NBLST2; NBPhox; neuroblastoma paired-type homeobox protein; Neuroblastoma Phox; paired like homeobox 2B; paired like homeobox2B; paired mesoderm homeobox 2b; Paired mesoderm homeobox protein 2B; Paired-like homeobox 2B; PHOX2B; PHOX2B homeodomain protein; PHX2B; PMX2B Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 35 SOURCE: Rabbit