CST, 83932S, Oct-4A (D6C8T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Oct4A mouse. Validated for WB,IP,IF,F,ChIP,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, Oct-4A (D6C8T) Rabbit Monoclonal Antibody (CST #83932) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:800 Flow Cytometry (Fixed/Permeabilized): 1:200 Chromatin IP: 1:50 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, CUT&RUN Specificity / Sensitivity Oct-4A (D6C8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total murine Oct-4A protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val80 of murine Oct-4A protein. Background Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7). Alternate Names NF-A3; Oct; Oct-; Oct-3; Oct-3/; Oct-3/4; Oct-4; Oct3; Oct3/; Oct3/4; Oct4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; octamer-binding transcription factor 4 alternative; Otf; Otf-; OTF-3; Otf-4; Otf3; Otf3-; Otf3-rs7; Otf3g; Otf4; PO5F1; POU domain class 5 transcription factor 1 transcript variant 2 oct4b'; POU domain class 5 transcription factor 1 transcript variant 3 oct4b; POU domain, class 5, transcription factor 1; Pou5f1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG