CST, 84039S, ELF3 (F8J2G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ELF3/ESE1b. Validated for WB,IP,IF,ChIP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, ELF3 (F8J2G) Rabbit Monoclonal Antibody (CST #84039) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP, Chromatin IP-seq Specificity / Sensitivity ELF3 (F8J2G) Rabbit Monoclonal Antibody recognizes endogenous levels of total ELF3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ELF3 protein. Background The E26 transformation-specific (ETS) transcription factors bind to a core 5'-GGAA/T-3' DNA motif and are known to regulate many biological processes, including cell proliferation, differentiation, apoptosis, hematopoiesis, and angiogenesis; they have also been described in pathological conditions, including cancer (reviewed in 1). ETS transcription factor E74-like factor 3 (ELF3), also called ESE-1, ESX, Jen, and ERT, belongs to a subtype of ETS proteins characterized by a predominant epithelial-specific expression profile (2-6). ELF3 shows widespread expression across tissues with high expression in the digestive tract. Targeted knockout of the ELF3 gene in mice results in lethality in about 30% of the embryos to E11.5, along with abnormalities in the intestinal epithelium (7). ELF3 can have dualistic roles in cancer with tumor-promoting and suppressing activity (reviewed in 8,9). Dysregulation of ELF3 through genetic and epigenetic mechanisms such as inactivating mutations, promoter methylation, and gene amplification have been reported across multiple cancer types and stages of disease. In addition, ELF3 has been shown to play a role in immune disorders and inflammation (10-12). Alternate Names E74 like ETS transcription factor 3; E74-like factor 3; E74-like factor 3 (ets domain transcription factor, epithelial-specific ); E74-like factor 3 (ETS domain transcription factor, serine box, epithelial-specific); E74-like factor 3 (ets domain transcription factor); ELF3; Epithelial-restricted with serine box; Epithelium-restricted Ets protein ESX; Epithelium-specific Ets transcription factor 1; EPR-1; ERT; ESE-1; ESX; ets domain transcription factor, serine box (epithelial-specific); ETS-related transcription factor Elf-3; JEN Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG