CST, 8408T, RSK1 (D6D5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RSK1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, RSK1 (D6D5) Rabbit Monoclonal Antibody (CST #8408) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 51169 . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RSK1 (D6D5) Rabbit Monoclonal Antibody recognizes endogenous levels of total RSK1 protein. This antibody does not cross-react with other RSK isoforms. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu718 of human RSK1 protein. Background The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). Alternate Names 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); HU-1; KS6A1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPK1; MAPKAPK1A; p90-RSK 1; p90RSK; p90RSK1; p90S6K; ribosomal protein S6 kinase A1; ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RPS6KA1; RSK; RSK-1; RSK1; S6K-alpha 1; S6K-alpha-1 Specification REACTIVITY: H M R Mk B Pg SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG