CST, 84149T, Procathepsin L (E9B2U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Cathepsin L. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Procathepsin L (E9B2U) Rabbit Monoclonal Antibody (CST #84149) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Procathepsin L (E9B2U) Rabbit Monoclonal Antibody recognizes endogenous levels of total procathepsin L protein. This antibody detects the procathepsin L proprotein, but does not detect the cleaved (proteolytically active) form of cathepsin L protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu110 of human procathepsin L protein. Background Cathepsin L belongs to the C1 family of peptidases, a large and diverse family of cysteine (thiol) proteases that play fundamental roles in protein processing and degradation. The catalytic activity of C1 peptidases maps to a nucleophilic cysteine thiol, usually found within a catalytic dyad or triad (1). Cathepsin L, initially synthesized as procathepsin L before undergoing proteolytic cleavage to its active form, is a ubiquitously expressed thiol protease, expressed primarily in lysosomes but which is also secreted by some cell types. Its diverse functions include general protein catabolism and autophagy (2), antigen processing (3), promoting angiogenesis (4), and extracellular matrix degradation (5,6). An isoform of cathepsin L has also been reported in the nucleus, where it was shown to be involved in the regulation of transcription factor activity (7). Targeted deletion of the gene in mice embryos was non-lethal, but resulted in periodic hair loss and skin defects, including epidermal hyperplasia, acanthosis, and hyperkeratosis (8). More recently, research studies have reported that cathepsin L is involved in viral protein processing during viral replication, and that cathepsin L inhibitors may have utility in combating infection by SARS-CoV-2 and related coronaviruses (9). Alternate Names cathepsin L; Cathepsin L1; Cathepsin L1 heavy chain; Cathepsin L1 light chain; CATL; CATL1; CTSL; CTSL1; FLJ31037; Major excreted protein; MEP; Procathepsin L Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG