CST, 84298S, CD62P/P-Selectin (E8Q1W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SELP. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD62P/P-Selectin (E8Q1W) Rabbit Monoclonal Antibody (CST #84298) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:400 - 1:1600 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier free (BSA and azide free) version of this product see product # 31214 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD62P/P-Selectin (E8Q1W) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD62P/P-Selectin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu433 of human CD62P/P-Selectin protein. Background CD62P/P-Selectin is a type-I transmembrane glycoprotein that is largely expressed and stored in intracellular granules by resting platelets, megakaryocytes, and vascular endothelial cells (1,2). The restricted expression pattern of CD62P/P-Selectin positions this molecule as a key regulator of hemostasis, thrombosis, and inflammation. Platelets and endothelial cells that become activated in response to proinflammatory cytokines express CD62P/P-Selectin on the cell surface, which serves as an adhesion receptor to facilitate recruitment of leukocytes to sites of inflammation (3-5). CD62P/P-Selectin has also been implicated in cancer cell metastasis by promoting the adhesion of tumor cells in circulation to activated platelets and endothelial cells (6,7). Alternate Names CD62; CD62 antigen-like family member P; CD62P; FLJ45155; GMP-140; GMP140; GMRP; Granule membrane protein 140; granule membrane protein 140kDa; granulocyte membrane protein; GRMP; LECAM3; Leukocyte-endothelial cell adhesion molecule 3; LYAM3; P-selectin; PADGEM; Platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; PSEL; selectin P; selectin P (granule membrane protein 140kDa, antigen CD62); SELP Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG