CST, 84309S, Phospho-FGF Receptor 1 (Tyr766) Antibody

Polyclonal Antibody for studying FGFR1 (Tyr766) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-FGF Receptor 1 (Tyr766) Antibody (CST #84309) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-FGF Receptor 1 (Tyr766) Antibody recognizes endogenous levels of FGFR1 protein only when phosphorylated at Tyr766 of human FGF Receptor 1. The antibody may cross react with other phosphorylated receptor tyrosine kinases such as EGFR/HER2/PDGFR. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr766 of human FGF Receptor 1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand-binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components, such as Crk and PLCγ (4,5). Alternate Names Basic fibroblast growth factor receptor 1; bFGF-R-1; BFGFR; CD331; CEK; ECCL; FGFBR; FGFR-1; FGFR1; FGFR1/PLAG1 fusion; Fibroblast growth factor receptor 1; FLG; FLJ99988; FLT-2; FLT2; Fms-like tyrosine kinase 2; fms-related tyrosine kinase 2; HBGFR; heparin-binding growth factor receptor; HH2; HRTFDS; hydroxyaryl-protein kinase; KAL2; N-SAM; OGD; Proto-oncogene c-Fgr; soluble FGFR1 variant 1; soluble FGFR1 variant 2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120, 145 SOURCE: Rabbit