CST, 84360SF, SGO2 (E9K4N) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Sgo2. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, SGO2 (E9K4N) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #84360) is ready to ship. Product Usage Information This product is the carrier free version of product #50616. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 50616 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SGO2 (E9K4N) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SGO2 protein. Based on the sequence of the immunogenic peptide, this antibody is expected to recognize isoforms 1-3 of human SGO2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly15 of human SGO2 protein. Background SGO2 (Shugoshin-2, SGOL2) is a centromeric protein required for the protection of cohesion complexes in meiosis (1). In mitosis, SGO2 and MAD2 regulate separase activity in the absence of securin to ensure appropriate timing of chromosome segregation (2). SGO2 is highly expressed in prostate cancer, and it promotes proliferation and migration by stabilizing the small GTPase Rab1A (3). High SGO2 expression in hepatocellular carcinoma (HCC) predicts poor overall survival (4), promoting tumor proliferation by regulating MAD2 expression and function (5). Alternate Names FLJ25211; MGC129665; Sgo2; SGOL2; Shugoshin 2; Shugoshin-2; Shugoshin-like 2; shugoshin-like 2 (S. pombe); Tripin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 190 Source/Isotype: Rabbit IgG