CST, 84390S, Neurofilament-M Antibody

Polyclonal Antibody for studying Neurofilament-M. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Neurofilament-M Antibody (CST #84390) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Neurofilament-M Antibody recognizes endogenous levels of total Neurofilament-M protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human Neurofilament-M protein. Antibodies are purified by peptide affinity chromatography. Background The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders, including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1). Alternate Names 160 kDa neurofilament protein; NEF3; NEFM; Neurofilament 3; Neurofilament M; neurofilament medium; Neurofilament medium polypeptide; Neurofilament triplet M protein; neurofilament-3 (150 kD medium); neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; NF-M; NFM Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 160 SOURCE: Rabbit