CST, 8442S, SLC1A4 Antibody

Polyclonal Antibody for studying SLC1A4. Validated for Western Blotting. Highly specific and rigorously validated in-house, SLC1A4 Antibody (CST #8442) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SLC1A4 Antibody recognizes endogenous levels of total SLC1A4 protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SLC1A4 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background SLC1A4, also known as ASCT1, is a neutral amino acid transporter. Its other name, ASCT1, was given because it mediates obligatory exchange of alanine, serine, cysteine, and threonine (1). SLC1A4 mediates the efflux of glutamate from the neuron into the synaptic junction via calcium-independent release, as well as mediating the efflux of L-serine from glial cells and its uptake by neurons (2). SLC1A4-mediated transport is shown to involve a symmetrical potassium-independent electroneutral exchange of neutral amino acids and sodium, such that the current activated during transport is carried only by chloride ions (3). Alternate Names Alanine/serine/cysteine/threonine transporter 1; ASCT-1; ASCT1; glutamate/neutral amino acid transporter; Neutral amino acid transporter A; SATT; SLC1A4; solute carrier family 1 (glutamate/neutral amino acid transporter), member 4; Solute carrier family 1 member 4; SPATCCM Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 60-80 SOURCE: Rabbit