CST, 8444S, HSPA8 (D12F2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HSC70. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, HSPA8 (D12F2) Rabbit Monoclonal Antibody (CST #8444) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HSPA8 (D12F2) Rabbit Monoclonal Antibody recognizes endogenous levels of total HSPA8 protein. This antibody cross-reacts with HSPA2, HSPA1A, and HSPC70. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys25 of human HSPA8 protein. Background HSPA8, alternately known as HSC70 or HSP73, is a constitutively expressed member of the HSP70 superfamily (1). Although its primary role in cells appears to be that of a general chaperone for unfolded proteins, HSPA8 has also been identified as the uncoating ATPase responsible for removing clathrin from coated vesicles and may also play a role in stabilizing untranslated mRNAs (1-5). In addition to these "housekeeping" functions, HSPA8 may also have an important role in inducible cellular stress responses. For example, oxidative or thermal stress promotes the nuclear/nucleolar accumulation of HSPA8, where it forms a complex with the topoisomerase I complex and likely protects it from heat inactivation (6,7). HSPA8 is reportedly phosphorylated in response to DNA damage, but it remains unclear what effect, if any, this has on HSPA8 function (8-10). Numerous high throughput studies support this observation. For more information, please see the HSPA8 page in PhosphoSitePlus at www.phosphosite.org. Alternate Names constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kD protein 8; heat shock 70kDa protein 8; Heat shock cognate 71 kDa protein; heat shock cognate protein 54; heat shock cognate protein, 71-kDa; heat shock protein family A (Hsp70) member 8; HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; HSP71; HSP73; HSP7C; HSPA10; HSPA8; LAP-1; LAP1; lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; N-myristoyltransferase inhibitor protein 71; NIP71 Specification REACTIVITY: H M R Mk B Pg SENSITIVITY: Endogenous MW (kDa): 70-72 Source/Isotype: Rabbit IgG