CST, 8446S, R-Ras Antibody

Polyclonal Antibody for studying RRas. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, R-Ras Antibody (CST #8446) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity R-Ras Antibody recognizes endogenous levels of total R-Ras protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human R-Ras protein. Antibodies are purified by protein A and peptide affinity chromatography. Background R-Ras, a member of the small GTPase family, is homologous to H-, K- and N-Ras, but does not activate MAP kinase pathways and is only weakly oncogenic (1). Instead, R-Ras is engaged in integrin activation (2). The effector loop and the carboxy-terminal proline-rich and prenylation sites of R-Ras are critical for integrin activation (3,4). Phosphorylation by EphB2 receptor tyrosine kinase and Src at Tyr66 of R-Ras suppresses integrin activity (5,6). Alternate Names Oncogene RRAS; p23; R-Ras; ras family small GTP binding protein R-Ras; RAS related; Ras-related protein R-Ras; related RAS viral (r-ras) oncogene homolog; RRAS Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 24 SOURCE: Rabbit