CST, 84476S, SOAT1 (E5O4K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SOAT1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SOAT1 (E5O4K) Rabbit Monoclonal Antibody (CST #84476) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SOAT1 (E5O4K) Rabbit Monoclonal Antibody recognizes endogenous levels of total SOAT1 protein. The higher molecular weight bands observed at 90 kDa, 135 kDa, and 185 kDa in the IP experiment are probably due to the oligomer formation under the IP lysate condition. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SOAT1 protein. Background SOAT1 (Sterol O-acyltransferase 1; ACAT1) is an O-acyltransferase that functions in the endoplasmic reticulum (ER) to catalyze the formation of cholesterol esters from free cholesterol and long chain fatty acyl-coenzyme A. The cholesterol esters are incorporated into cytoplasmic lipid droplets, thereby preventing excess free cholesterol from inducing lipid-mediated cell toxicity, including ER stress (1). Research studies have shown that pharmacological inhibition of SOAT1 in tumor cells induced lipid-mediated cell toxicity that suppressed tumor cell growth and promoted tumor cell apoptosis (2,3). Pharmacological SOAT1 inhibition was also shown to stimulate autophagy-mediated proteolysis in microglia, leading to enhanced clearance of amyloid peptide Aβ42 (4,5). Collectively, these findings suggest that SOAT1 inhibition may have therapeutic potential in both cancer and Alzheimer's disease. Alternate Names ACACT; ACACT1; ACAT; ACAT-1; ACAT1; acyl-Coenzyme A: cholesterol acyltransferase; Acyl-coenzyme A:cholesterol acyltransferase 1; Cholesterol acyltransferase 1; RP11-215I23.2; SOAT; SOAT1; STAT; sterol O-acyltransferase (acyl-Coenzyme A: cholesterol acyltransferase) 1; Sterol O-acyltransferase 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG