CST, 84478T, MAT2A (E5I7C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MAT2A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MAT2A (E5I7C) Rabbit Monoclonal Antibody (CST #84478) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MAT2A (E5I7C) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAT2A protein. This antibody does not cross-react with MAT1A protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAT2A protein. Background Methionine adenosyltransferase II alpha (MAT2A) uses methionine and ATP as substrates to generate S-adenosylmethionine (SAM), a universal methyl group donor in cells. Tumor-initiating cells (TICs) have high methionine cycle activity mediated by MAT2A, leading to their metabolic dependency on exogenous methionine (1). Studies show that mTORC1 signaling induces MAT2A expression, leading to elevated synthesis of SAM. In addition, mTORC1 increases the protein level of Wilms' tumor 1-associating protein (WTAP), a positive regulatory subunit of the human N -methyladenosine (m A) RNA methyltransferase complex. mTORC1, therefore, promotes N -methylation of mRNA to stimulate protein synthesis and cell growth (2). Furthermore, inhibition of MAT2A activity was shown to decrease proliferation of cancer cells deficient in methylthioadenosine phosphorylase (MTAP) (3). Alternate Names AdoMet synthase 2; adoMet synthetase 2; AMS2; MAT 2; MAT-II; MAT2A; MATA2; MATII; Methionine adenosyltransferase 2; methionine adenosyltransferase 2A; Methionine adenosyltransferase II; methionine adenosyltransferase II, alpha; METK2; S-adenosylmethionine synthase isoform type-2; S-adenosylmethionine synthetase isoform type-2; SAMS2; testicular tissue protein Li 121 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 47, 49 Source/Isotype: Rabbit IgG