CST, 84487SF, c-Jun (L34E12) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying JUN. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, c-Jun (L34E12) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #84487) is ready to ship. Product Usage Information This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity c-Jun (L34E12) Mouse Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of c-Jun. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Isolated from a strain of E. coli that carries the coding sequence for human c-Jun residues 1-89 (kindly provided by Dr. J.R. Woodgett). Protein was purified by affinity chromatography. Background c-Jun is a member of the Jun family containing c-Jun, JunB, and JunD, and is a component of the transcription factor activator protein-1 (AP-1). AP-1 is composed of dimers of Fos, Jun, and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals, including growth factors, chemokines, and stress, activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knockout studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes, including axon regeneration (4), liver regeneration (5), and T cell development (6). AP-1 regulated genes exert diverse biological functions, including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival, as well as hypoxia and angiogenesis (8,10). Research studies have implicated c-Jun as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, and rheumatoid arthritis (11,12). Alternate Names Activator protein 1; AP-1; AP1; c-Jun; cJUN; enhancer-binding protein AP1; JUN; Jun activation domain binding protein; jun oncogene; Jun proto-oncogene, AP-1 transcription factor subunit; p39; Proto-oncogene c-Jun; proto-oncogene cJun; Transcription factor AP-1; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 43, 48 Source/Isotype: Mouse IgG1