CST, 8449S, StAR (D10H12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying StAR. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, StAR (D10H12) Rabbit Monoclonal Antibody (CST #8449) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity StAR (D10H12) Rabbit Monoclonal Antibody recognizes endogenous levels of total StAR protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg272 of human StAR protein. Background Steroidogenic acute regulatory protein (StAR) plays a significant role in cholesterol transport from the cytoplasmic outer membrane to the inner mitochondrial membrane (1). The 37 kDa precursor is cleaved to generate an active 28 kDa protein capable of facilitating cholesterol metabolism into pregnenolone (2,3). StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue (3). Abnormalities in StAR gene expression are impacted in autosomal Lipoid Congenial Adrenal Hyperplasia (LCAH) resulting in defects in pregnenolone and cortisol synthesis (4). The mechanism of cholesterol binding to StAR has yet to be elucidated (4). Alternate Names cholesterol trafficker; mitochondrial steroid acute regulatory protein; STAR; StAR related lipid transfer (START) domain containing 1; StAR-related lipid transfer (START) domain containing 1; STARD1; START domain containing 1; START domain-containing protein 1; steroid acute regulatory protein; steroidogenic acute regulator; steroidogenic acute regulatory protein; Steroidogenic acute regulatory protein, mitochondrial; testis secretory sperm-binding protein Li 241mP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG